Objective

To display the crystal structure of a G-protein coupled receptor.

Background

Using ICM it is easy to quickly search and download PDB files using the .pdb search. tab.

Instructions

1. Click on the PDB Search Tab
2. Type .bovine rhodopsin. into the search box and click the button next to it. A table of hits will be displayed at the bottom of the GUI.
3. Click on the ID field of structure 1F88 to display the structure.
4. Double click on the head field to display the XPDB structure and fully download the PDB coordinates.

Notes and things to try:

• Try searching for a PDB file by sequence or homology. Use the drop down menu next to the PDB search box to define which kind of search you are undertaking.
• XPDB is a unique database developed by Molsoft which displays SWISSPROT annotation onto protein structures.
• In order to fully transfer the coordinates from the PDBSearchResults table you need to double click or else the PDB will be highlighted as a TempMinObject. Alternatively, right click menu in ICM workspace gives an option .Read Full PDB Entry.

Manual References (Web Links)

Finding a PDB Structure