To generate a stack of energetically favorable protein conformers using the ICM Biased Probability Monte Carlo method:

• Read in a protein structure and convert to an ICM object.
• Select the residues you wish to sample.
• MolMechanics/Sample Protein
• Enter a value for Effort. This represents the length of the simulation - values between 1 and 10 will give a thorough sampling. A larger effort value will be needed for larger proteins.
• Enter a value for the Angular Vicinity (default 15). Please see http://www.molsoft.com/man/reals.html#vicinity
• Enter a Temperature value. Please see http://www.molsoft.com/man/reals.html#temperature
• Click OK.
• When the simulation is finished a stack of conformations will be displayed in the ICM workspace.