Jul 23 2008
Contents
 
Introduction
Overview - RECOMMENDED READING FOR NEW ICM USERS
File Menu
Graphics Move Tools
Display Tab
 Hydrogens
 Structure Representation
  Wire
  Stick/Ball
  Ribbon
  Skin
  CPK
  Surface
 H-Bond
 Coloring
 Background
 Labeling
 Tethers
 Dist/Restraints
 Superimpose
 Display Clash
Light Tab
Labels Tab
PDB Search Tab
Meshes Tab
View Menu
Selections
Tables
Local DB
Sequences
Bioinfo Menu
Tools Menu - Xray
Tools Menu - 3D Predict
Tools Menu - Analysis
Tools Menu - Superimpose
Homology and Modelling
Working with Chemistry Tools
Chemsitry Menu
Docking
Ligand Editor
Animations, Slides, & Documents
ActiveICM
Movie Making
Frequently Asked Questions
Tutorial - Graphical Display
Molecular Document
Tutorial - Working with PDB Protein Structures
Tutorial - Working with Sequence Alignments
Tutorial - Ligand Binding Pocket Analysis
Tutorial - Homology and Modeling Tools
Tutorial - Crystallographic Analysis Tools
Tutorial - Working with Chemical Tables
Tutorial - Working with the Molecular Editor
Tutorial - Chemical Searching
Tutorial - Docking and Virtual Ligand Screening
 
Index
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5.2 Structure Representation
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[ Wire | Stick/Ball | Ribbon | Skin | CPK | Surface ]

There are six main types of structural representation in ICM. They are wire, ball and stick (Xstick), ribbon, skin, CPK and dot envelope (surface).

To display one of these representations:

  • Click on the representation button you desire in the display tab.

To remove a displayed representation or to toggle between display and undisplay:

  • Click on the corresponding representation button in the display tab.

NOTE: The button display will change appearance slightly when pressed. This makes it easier to identify which representations are currently being displayed.

Many characteristics of the graphical representation such as color can be changed by clicking and holding on the button or by cliking the plus(+) and minus(-) buttons next to them.

Wire : Thin Wire : Normal Wire : Thick
Wire : Chemistry Wire : Tree Xstick- Thin
Xstick : Thick Xstick : Stick / Ball Ribbon : Ribbon
Ribbon : Segment Ribbon : Protein Worm Ribbon : Transparent
CPK : Default Skin : Default Skin : Transparent
Surface : Tight Surface : Normal Surface - Sparse

5.2.1 Wire Representation


Click and hold on the wire representation button. A menu will be displayed as shown below.

To change the wire style:

  • Click and hold on the wire representation button and then click on wire, chemistry or tree.

To change the size of the wire representation:

  • Click and hold on the wire representation button and then click on thin, normal or thick.

NOTE: Clicking on the +/- next to the wire representation button also changes the thickness of the wire representation.

To undisplay representations other than wire:

  • Click and hold on the wire representation button and then click on undisplay other representations.

If you make a mistake or you are not happy with the way your structure is displayed with the wire representation:

  • Click and hold on the wire representation button and then click on reset to default.

5.2.2 Stick and Ball (Xstick) Representation


Click and hold on the stick and ball representation button. A menu will be displayed as shown below.

To change the style of the Xstick representation:

  • Click and hold on the stick and ball representation button and then click on style. Choose a style as shown below.

To change the size of the Xstick representation:

  • Click and hold on the stick and ball representation button and then click on set thickness and set ball/stick ratio

NOTE: Clicking on the arrow next to the Stick and ball representation button can also change the thickness of the xstick representation.

In order to make some parts of your picture clearer, the xstick representation can be set to transparent:

  • Click and hold on the stick and ball representation button and then click on transparent.

To undisplay representations other than xstick:

  • Click and hold on the stick and ball representation button and then click on undisplay other representations.

If you make a mistake or you are not happy with the way your structure is displayed with the xstick representation:

  • Click and hold on the stick and ball representation button and then click on reset to default.

5.2.3 Ribbon Representation


Click and hold on the ribbon representation button. A menu will be displayed as shown below.

To change the style of the Ribbon representation:

  • Click and hold on the ribbon representation button and then click on a style option.

To accurately represent the secondary structure of the molecule in ribbon representation you may wish to assign secondary structure:

  • Click and hold on the ribbon representation button and then click on assign sec. structure. New in version 3.5-2 is they smooth ribbon style.

To make some parts of your picture clearer, the ribbon representation can be set to transparent:

  • Click and hold on the ribbon representation button and then click on transparent.

To undisplay representations other than ribbon:

  • Click and hold on the ribbon representation button and then click on undisplay other representations.

If you make a mistake or you are not happy with the way your structure is displayed with the ribbon representation:

  • Click and hold on the ribbon representation button and then click on reset to default.

NOTE: Always use the ICM assign sec.** structure tool in the ribbon right click menu to get accurate secondary structure assignment. This is particularly important when studying helices which may have non-cannonical elements within them such as 3/10 or pi. To view non-cannonical helix segments use the segment option in the ribbon right click menu.

The helices in rhodopsin and bacteriorhodopsin are shown below in segment representation. Non-cannonical regions are represented as breaks in the helix segment.

To change the display of chain breaks:

  • Click and hold on the ribbon represenation button.
  • Select the options Display Chain Breaks and Display Chain Break label.

5.2.4 Skin Representation


Click and hold on the skin representation button. A menu will be displayed as shown below.

To make some parts of your picture clearer, the skin representation can be set to tight, normal or sparse:

  • Click and hold on the skin representation button and then click on either tight, normal or sparse.

To undisplay representations other than skin:

  • Click and hold on the skin representation button and then click on undisplay other representations.

If you make a mistake or you are not happy with the way your structure is displayed with the skin representation:

  • Click and hold on the skin representation button and then click on ** reset to default**.

NOTE: Sometimes due to singularity problems holes may appearwithin the skin surface. To cure this infliction select atoms nearby and right click select Advanced->RandomizeAtoms

5.2.5 CPK Representation


Click and hold on the CPK representation button. A menu will be displayed as shown below.

To undisplay representations other than CPK:

  • Click and hold on the CPK representation button and then click on undisplay other representations.

If you make a mistake or you are not happy with the way your structure is displayed with the cpk representation.

  • Click and hold on the CPK representation button and then click on reset to default.

5.2.6 Surface Representation


Click and hold on the surface representation button. A menu will be displayed as shown below.

To change the style of the surface representation:

  • Click and hold on the surface representation button and then click on tight, normal, or surface.

To undisplay representations other than surface:

  • Click and hold on the surface representation button and then click on undisplay other representations.

If you make a mistake or you are not happy with the way your structure is displayed with the surface representation:

  • Click and hold on the surface representation button and then click on reset to default.


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